Rat Nasal Respiratory Mucosa-Derived Ectomesenchymal Stem Cells Differentiate into Schwann-Like Cells Promoting the Differentiation of PC12 Cells and Forming Myelin In Vitro

Figure 6

Western blot and immunofluorescent labeling indicated that SC-like cells promoted the differentiation of PC12 cells into mature neuron-like cells. (a) Neural cell markers including NF-H, GAP-43, PSD-95, and Synapsin II were detected in PC12 cells after being cultured for 5 days for all four groups, which were PC12 cells treated with DF12 medium, PC12 cells treated with SCDM, PC12 cells treated with DF12 medium and REMSCs, and PC12 cells treated with SC-like cells and SCDM. β-Actin was used as a loading control. The experiments were replicated three times and a representative blotting was shown. Each bar showed the ratio of marker protein to β-actin. The data were presented as the mean ± SEM of three independent experiments. represent significant differences compared with group A; ^## represent significant differences compared with group C. Immunofluorescent staining showed that differentiated PC12 cells in group D expressed NF-H (b) and PSD-95 (c). Bar: 50 μm for all pictures.

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