Research Article

The Organotypic Longitudinal Spinal Cord Slice Culture for Stem Cell Study

Figure 4

The intrinsic spinal cord axons forming a fiber tract. Two weeks after SCC preparation the anatomy of cultured slices was visualized by live imaging in light converted microscope (a) and by immunohistochemical analysis using neuronal and astrocytes markers ((b)–(d)). The scale bar is the equivalent of 200 μm.