Table of Contents Author Guidelines Submit a Manuscript
Stem Cells International
Volume 2015 (2015), Article ID 471216, 10 pages
http://dx.doi.org/10.1155/2015/471216
Research Article

The Organotypic Longitudinal Spinal Cord Slice Culture for Stem Cell Study

1NeuroRepair Department, Mossakowski Medical Research Centre, Polish Academy of Sciences, 5 Pawinskiego Street, 02-106 Warsaw, Poland
2Molecular Biology Unit, Mossakowski Medical Research Centre, Polish Academy of Sciences, 5 Pawinskiego Street, 02-106 Warsaw, Poland
3Translative Platform for Regenerative Medicine, Mossakowski Medical Research Centre, Polish Academy of Sciences, 5 Pawinskiego Street, 02-106 Warsaw, Poland
4Stem Cell Bioengineering Laboratory, Mossakowski Medical Research Centre, Polish Academy of Sciences, 5 Pawinskiego Street, 02-106 Warsaw, Poland

Received 3 November 2014; Revised 29 December 2014; Accepted 6 January 2015

Academic Editor: Hyoungshin Park

Copyright © 2015 Joanna Sypecka et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

The adult slice culture survives about one week maintaining proper parallel fiber tract and proliferating ability. Most of the cells were NG2 positive, but also TUJ/NF200 expressing cells were present. The astrocytes and microglia populations, in the neonatal and adult organotypic spinal cord slice cultures were comparable.

Supplementary Figure 1: The longitudinal spinal cord slice culture derived from adult rats. 7 days after SCC preparation the anatomy of cultured slices was visualized by immunohistochemical analysis using neuronal (β- tubulin III), astrocytic (GFAP), oligodendrocyte (NG2) markers, as well as Ki67 – a marker of proliferating cells (A-D). The scale bar is the equivalent of 200 µm. Cell nuclei (blue) were visualized by Hoechst 33258.

  1. Supplementary Figure