Research Article

Rho/MRTF-A-Induced Integrin Expression Regulates Angiogenesis in Differentiated Multipotent Mesenchymal Stem Cells

Figure 3

MRTF-A activates the transcription of integrins α1 and α5 by binding to the CArG box with their promoters. (a and b) Luciferase assays were performed 24 h after transfection of MRTF-A and integrin α1/integrin α5 promoter-luc plasmids into MSCs, COS-7 cells and HUVECs. ; , . (c and d) ChIP assays were performed in MSCs treated with VEGF at d2, d4, and d7. Cross-linked chromatin was immunoprecipitated with specific anti-MRTF-A antibody. The precipitated chromatin DNA was then purified and amplified by reverse transcription PCR (c) and real time PCR (d) with specific primers that spanned CArG boxes in integrin promoters. The negative control in the immune-precipitation was performed with IgG antibody.