Research Article
Rho/MRTF-A-Induced Integrin Expression Regulates Angiogenesis in Differentiated Multipotent Mesenchymal Stem Cells
Figure 5
Knocking-down MRTF-A obstructed angiogenesis and migration in HUVECs by affecting integrin expression. (a) Western blotting confirmed the inhibition of endogenous MRTF-A in HUVECs transfected with siMRTF-A. (b) The capillary-like structures formed by HUVECs were observed on Matrigel. (c) Quantification of the capillary-like structures. , . The migratory ability of HUVECs transfected with siMRTF-A or control siRNA was determined by wound healing (d) and transwell chamber assays (e). (f) Cell migration was quantified by calculating relative cell numbers. , . (g) After transfection with siMRTF-A or control siRNA, HUVECs were treated with VEGF for 24 h, and the expression of integrins α1, α5, and β1 was then estimated by real-time PCR. , . (h) Western blotting for integrins α1, α5 and β1. (i) The relative quantification of the protein expression. , .
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