Human Umbilical Cord Mesenchymal Stem Cells: A New Therapeutic Option for Tooth Regeneration

Figure 1

Isolation, culture, and identification of hUCMSCs. (a) Primary mesenchymal stem cells isolated from human Wharton’s jelly formed colonies after 7 days, reached confluency after 10 days, and were in a vortex-like arrangement after 14 days. (b) The adherent cells were stained positively for mesenchymal markers CD29, CD44, and CD105 but were negative for hematopoietic lineage markers CD34 and CD45 and endothelial cell marker CD31. (c) After adipogenic induction for 2 weeks, vacuole-like changes were observed under a microscope (left, ×40), and the cells were stained by oil red O (right). (d) After osteogenic induction, refractile substances were observed in cell colonies (left, ×40), and small round nodules were detected by Alizarin red S staining (right).

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