Human Umbilical Cord Mesenchymal Stem Cells: A New Therapeutic Option for Tooth Regeneration

Figure 2

TGC-CM induces hUCMSCs into odontoblast-like cells in vitro. (a) After hUCMSCs were induced in TGC-CM for 3 days, there was little change in the cell morphology. After 14 days, the TGC-CM-induced hUCMSCs grew well and calcified areas were observed (arrow), but the morphology was similar to uninduced cells. (b) Odontoblast marker proteins DSP and DMP-1 were detected in TGC-CM-induced hUCMSCs by immunocytochemistry, but not in uninduced hUCMSCs. (c) Western blotting showed that TGC-CM-induced hUCMSCs and pulp tissue expressed DMP-1 and DSP, whereas uninduced hUCMSCs did not express these proteins. (d) and (e) Relative mRNA levels of DMP-1 and DSPP were determined by quantitative PCR.

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