Human Umbilical Cord Mesenchymal Stem Cells: A New Therapeutic Option for Tooth Regeneration

Figure 3

Preparation and identification of hTDM. (a) The gross appearance as well as HE staining of hTDM was observed under a microscope at various magnifications (×40, ×100, and ×400). HE staining showed the loose fiber tracts on the surface of the prepared hTDM. (b) Masson’s tricolor staining showed dark red with gradual darker blue from distal to proximal pulp cavity dentin, where collagen fibers existed from low to high abundance in hTDM (right), just like staining in normal tooth root (left). (c) Scanning electron microscopic observation of the prepared hTDM. (d) Detection of DSP and DMP-1 in hTDM by immunohistochemistry. (e) Growth curves of hTDM-induced hUCMSCs and normal cultured hUCMSCs without hTDM as determined by MTT assays. (f) Observation of hUCMSCs after induction with hTDM for 2 hours, 24 hours, and 7 days under a scanning electron microscope.

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