|
| | Animal model | Defect type | Delivery method | ADSCs processing | Study design | Results |
|
|
Nathan et al. 2003, Tissue Engineering [22] | Rabbit | Osteochondral defect | Surgical implantation | Expanded homologous | (i) ADSCs + fibrin
(ii) Periosteum MSCs + fibrin
(iii) Fibrin only | Better defect reconstruction in experimental groups than in control, with superior results for ADSCs |
|
| Masuoka et al. 2006, J Biomed Mater Res [41] | Rabbit | Osteochondral defect | Surgical implantation | Expanded autologous | (i) ADSCs + ACHMS-scaffold
(ii) ACHMS-scaffold alone
(iii) Empty defect | New regenerated hyaline-like cartilage only in the ADSCs group |
|
| Dragoo et al. 2007, Tissue Eng [36] | Rabbit | Osteochondral defect | Surgical implantation | Expanded autologous | (i) ADSCs + fibrin glue matrix
(ii) Empty defect | 100% of defects healed in ADSCs group and only 8% in control group |
|
| Koga et al. 2008, Cell Tissue Res [24] | Rabbit | Osteochondral defect | Surgical implantation | Expanded homologous | (i) ADSCs
(ii) BMSCs
(iii) Synovium MSCs
(iv) Muscle MSCs | Great amount of cartilage matrix production in BMSCs and synovium MSCs compared to other groups
Low chondrogenic potential for ADSCs |
|
| Zhang et al. 2009, Chin J Traumatol [40] | Rabbit | Osteochondral defect | Surgical implantation | Expanded homologous | (i) ADSCs + calcium alginate gel
(ii) Calcium alginate gel alone
(iii) Empty defect | Complete defect healing only in the ADSCs group |
|
| Frisbie et al. 2009, J Orthop Res [27] | Horse | Induced OA (by arthroscopy) | Injective | Expanded autologous | (i) ADSCs
(ii) BMSCs
(iii) Placebo (sham surgery) | No adverse treatment-related events
ADSCs decreased synovial fluid PDE G2
Overall improvement with cells n.s. |
|
| Cui et al. 2009, Biomaterials [37] | Pig | Osteochondral defect | Surgical implantation | Expanded autologous chondrogenic-induced | (i) ADSCs + PGA/PLA
(ii) PGA/PLA alone | Hyaline-like neo-cartilage
Well-integrated implant
Lack of cartilage repair for acellular group |
|
| Oliveira et al. 2010, J Orthop Res [45] | Rabbit | Osteochondral defect | Surgical implantation | Expanded autologous chondrogenic-induced | (i) Predifferentiated ADSCs + gellan gum (GG)
(ii) Not predifferentiated ADSCs + GG
(iii) Articular chondrocytes + GG
(iv) GG alone
(v) Empty defect | Good defect filling and integration with surrounding cartilage for cell-loaded constructs
Best results with predifferentiated ADSCs with GG |
|
| Im and Lee 2010, J Biomed Mater Res B Appl Biomater [23] | Rabbit | Osteochondral defect | Surgical implantation | Expanded autologous | (i) ADSCs + (PCL)/F127 scaffold
(ii) Scaffold alone
(iii) TGF-b2 + BMP-7 + scaffold
(iv) ADSCs + TGF-b2 + BMP-7 + scaffold | Incomplete cartilage healing for all groups
Better macroscopic score in growth factors groups compared to ADSCs alone |
|
| Li et al. 2011, Artif Cells Blood Substit Immobil Biotechnol [25] | Rabbit | Osteochondral defect | Surgical implantation | Expanded autologous | (i) ADSCs + DBM
(ii) BMSCs + DBM
(iii) Synovium MSCs + DBM
(iv) Periosteum MSCs + DBM
(v) Muscle MSCs + DBM
(vi) Empty defect | Good defect filling with new hyaline-like cartilage for each treatment group
BMSCs had best results for cartilage matrix synthesis, gross morphology, and histology |
|
| Toghraie et al. 2011, The Knee [32] | Rabbit | Induced OA
ACL transection
(ACLT) | Injective | Expanded homologous | (i) ADSCs from infrapatellar fat pad
(ii) Control (medium only) | Better cartilage quality, lower subchondral changes, and lower OA features for ADSCs group than controls |
|
| Toghraie et al. 2012, Arch Iran Med [33] | Rabbit | Induced OA
(ACLT) | Injective | Expanded homologous | (i) ADSCs from subcutaneous fat
(ii) Control (medium only) | Improved cartilage quality for ADSC group compared to control |
|
| Lee and Im 2012, Biomaterials [43] | Rat | Induced OA
(ACLT) | Injective | Expanded transduced SOX-trio genes autologous | (i) ADSCs transduced with SOX-trio genes + fibrin gel
(ii) ADSCs transfected with empty vector
(iii) Fibrin gel alone | Decrease of OA progression Decrease of OA progression for ADSCs in fibrin gel group |
| Surgical implantation | Good hyaline cartilage-like tissue regeneration for the transfected ADSCs/fibrin gel group
Incomplete healing and fibrous tissue in both controls |
|
| Ter Huurne et al. 2012, Arthritis Rheum [34] | Mouse | Induced OA (collagenase injection) | Injective | Expanded autologous | (i) ADSCs + 4% mouse albumin
(ii) Only 4% mouse albumin | Inhibition of OA progression in early stage of OA, whereas no effects with injections in late OA stage |
|
| Xie et al. 2012, Biomaterials [44] | Rabbit | Osteochondral defect | Surgical implantation | Expanded homologous | (i) ADSCs + PRP
(ii) BMSCs + PRP
(iii) PRP alone
(iv) Empty defect
(v) Sham treatment | Better repair for cell + PRP groups versus PRP alone
BMSCs higher chondrogenic differentiation
ADSCs differentiate into mature chondrocytes |
|
| Cao et al. 2012, J Biomed Biotechnol [58] | Rabbit | Osteochondral defect | Surgical implantation | Expanded autologous | (i) ADSCs-bBMPs-bilayered construct
(ii) bBMPs-bilayered construct
(iii) Empty defect | Better defect healing and integration with host tissue in the ADSCs-bBMPs-bilayered construct group |
|
| Desando et al. 2013, Arthritis Res Ther [35] | Rabbit | Induced OA
(ACLT) | Injective | Expanded autologous | (i) 2 mln ADSCs + 4% rabbit serum
(ii) 6 mln ADSCs + 4% rabbit serum
(iii) 4% rabbit serum alone
(iv) No treatment
(v) Sham group | Decrease of OA progression in the study groups compared to controls
Best results for implanted 2 mln ADSCs |
|
| van Pham et al. 2013, Stem Cell Res Ther [29] | Mouse | Induced OA (needle disruption) | Injective | Expanded human ADSCs | (i) ADSCS + 15% PRP
(ii) ADSCS + 10% FBS
(iii) PBS only
(iv) No treatment, no defect | Tendency for better cartilage repair improvement for cell-transplanted groups compared to PBS group, significantly better results for PRP-pretreated ADSCs on all parameters |
|
| van Pham et al. 2013, J Med Engineering [30] | Mouse | Induced OA (needle disruption) | Injective | Human SVF | (i) ADSCs + PRP
(ii) Only PBS
(iii) No treatment, no defect | Significant improvement in articular cartilage regeneration for PRP-pretreated ADSCs compared to PBS control
No adverse events, high safety |
|
| Jurgens et al. 2013, Bio Research [31] | Goat | Osteochondral defect | Surgical implantation | Expanded versus SVF autologous | (i) Expanded ADSCs + collagen I/III
(ii) Concentrated ADSCs + collagen I/III
(iii) Collagen I/III alone | Good tissue regeneration in all groups with tendency towards better results in cell-treated groups and in particular for concentrated ADSCs
No adverse events |
|
| Zhang et al. 2013, Acta Biomaterialia [39] | Rabbit | Osteochondral defect | Surgical implantation | Expanded chondrogenic-induced autologous | (i) Predifferentiated ADSCs + PLGA/CHI
(ii) PLGA/CHI alone | Good tissue regeneration, similar to healthy cartilage, well integrated with surrounding tissue in ADSCs/scaffold group compared to scaffold alone |
|
| Shi et al. 2013, Arthroscopy [42] | Rabbit | Osteochondral defect | Surgical implantation | Expanded and transfected autologous | (i) BMP-4 transfected ADSCs + PLLGA
(ii) Nontransfected ADSCs + PLLGA
(iii) PLLGA alone | Better improvement in chondrogenesis in vivo for the transfected ADSCs group with respect to untransfected and scaffold alone |
|
| Gong et al. 2014, Tissue Eng part A [38] | Pig | Osteochondral defect | Surgical implantation | Expanded chondrogenic-induced autologous | (i) ADSCs + PGA/PLA
(ii) PGA/PLA alone | Successful new cartilage
Graft remodeling between 3 and 6 m
Proteomic analysis detected differences versus native cartilage |
|
| Wang et al. 2014, Genet Mol Res [59] | Rabbit | Osteochondral defect | Surgical implantation | Expanded homologous | (i) ADSCs preseeded ACM
(ii) ACM only
(iii) Empty defect | Chondral tissue with hyaline features in ADSC-ACM group, with type II coll., fibrous tissue in ACM only |
|
| Ude et al. 2014, PLoS One [26] | Sheep | Induced OA
(ACLT) | Injective | Expanded chondrogenic-induced autologous | (i) ADSCs
(ii) BMSCs
(iii) Control | ADSCs had higher cell proliferation and BMSCs had better chondrogenic inductions and gene expressions
ICRS score n.s. ADSCs versus BMSCs |
|
