Development of a Xeno-Free Substrate for Human Embryonic Stem Cell Growth

Figure 5

Detection of specific markers of hESCs cultured on fixed xeno-free ECM after 25 passages. ((a), (c), (e), (g), (i), and (k)) hESCs nuclear staining with DAPI; ((b), (d), (f), (h), (j), and (l)) hESCs with staining of Oct4, Nanog, Sox2, SSEA4, Tra-1-60, and Tra-1-81, respectively.

(a) DAPI

(b) Oct4

(c) DAPI

(d) Nanog

(e) DAPI

(f) Sox2

(g) DAPI

(h) SSEA4

(i) DAPI

(j) Tra-1-60

(k) DAPI

(l) Tra-1-81