Stem Cells International

Review Article

Alkaline Phosphatase in Stem Cells

Table 2

Transcription factor binding site (TFBS) analysis of promoters of tissue nonspecific alkaline phosphatase isoforms.


Species	Gene symbol (name)	Matrix family/matrix
		V$HOXF	V$STEM		V$SORY	V$FKHD	V$RXRF
		V$NANOG.01	V$OCT3_4.02 (Oct-4)	V$OSNT.01	V$SOX2.01	V$HFH2.01 (FoxD3)	V$RAR_RXR.01

Human	ALPP (PLAP)	1	—	—	—	—	2
	ALPPL2 (GCAP)	—	1	1	1	—	1
	ALPL (TNAP)	1	3	4	—	—	3

Mouse	Alppl2 (EAP)	1	—	—	—	—	—
Mouse	Alpl (Akp2/TNAP)	1	6^*	2	1	1	7

NANOG, Oct3-4, SOX-2, FoxD3, and RAR/RXR binding motifs were predicted by the Genomatix software tool MatInspector (1) (digits denote numbers of matches) and further analysed using the rVista algorithm (based on Transfac Professional Library v10.2) (2) for evolutionary conserved motifs between mouse and human (grey fields). The asterisk “*” symbol indicates previously observed Oct-4 binding in Alpl (Akp2) regulatory sequences using the Chip-PET methodology (3, 4). Matrix V$OSNT.01 represents composed binding sites for Oct-4, Sox-2, Nanog, Tcf3 (Tcf7l1), and Sall4b in pluripotent cells.