Modulation of Hyaluronan Synthesis by the Interaction between Mesenchymal Stem Cells and Osteoarthritic Chondrocytes

Figure 4

Hyaluronan enzyme mRNA expression in BM-MSC after coculture. mRNA expression of hyaluronan synthase- (HAS-) 1, HAS-2, and HAS-3 (a-b) and hyaluronidase- (HYAL-) 1, HYAL-2, and HYAL-3 (c-d) after coculture relative to time-matched and cell-matched controls. Expression of HAS in cocultivated BM-MSC relative to BM-MSC monoculture after 3 days (a) and 6 days () (b); expression of hyaluronidases in cocultivated BM-MSC relative to BM-MSC monoculture after 3 days (c) and 6 days (d) (). Bars represent the mean fold change value ± SEM relative to BM-MSC in monoculture of hyaluronan enzymes. Statistical significance based on unpaired t-test was set according to the number of asterisks, as follows: , .

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