Stem Cells International / 2015 / Article / Fig 1

Research Article

A Double-Switch Cell Fusion-Inducible Transgene Expression System for Neural Stem Cell-Based Antiglioma Gene Therapy

Figure 1

Selection of glioma-specific promoter and NSC-specific miR. (a) Promoter activities of the lineage-specific promoter GFAP, tumor-specific promoter Survivin, and glioma-specific promoter HMGB2 compared to the strong universal promoter CMV in different NSC and glioma cell lines are quantified by luciferase assays. (b) Absolute expression levels of miR-199a/214 cluster members miR-199a-5p, miR-199a-3p, and miR-214 in different NSC and glioma cell lines are quantified by qPCR. miR copy numbers were calculated based on a standard curve generated using a synthetic LIN-4 RNA oligonucleotide. Error bars: s.d. , .
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