Targeted Knockdown of RNA-Binding Protein TIAR for Promoting Self-Renewal and Attenuating Differentiation of Mouse Embryonic Stem Cells

Figure 2

Downregulation of TIAR protein levels restrained the differentiation of E14 cells. (a) RT-PCR analysis of the gene expression of nestin, Pax6, Flk-1, brachyury, Sox17, and Gata6 on day 0 in TIAR RNAi E14 cells and pSIREN-RetroQ control E14 cells. E14 cells were used as control. (b) Quantitative RT-PCR analysis of the gene expression of nestin, Pax6, Flk-1, brachyury, Sox17, and Gata6 on day 4, day 8, and day 14 during EB differentiation in TIAR RNAi E14 cells and pSIREN-RetroQ control E14 cells. Day 4, day 8, and day 14 EB differentiation of E14 cells were used as control. (c) Western blot analysis of the gene expression of nestin, Flk-1, and Sox17 on day 4 during EB differentiation in TIAR RNAi E14 cells and pSIREN-RetroQ control E14 cells. (d) Western blot analysis of the gene expression of Flk-1 (day 4) and VE-cadherin (day 8) in monolayer differentiation of TIAR RNAi E14 cells and pSIREN-RetroQ control E14 cells. by one-way ANOVA. Control: pSIREN-RetroQ control E14 cells; TIAR RNAi: TIAR RNAi E14 cells.

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