Targeted Knockdown of RNA-Binding Protein TIAR for Promoting Self-Renewal and Attenuating Differentiation of Mouse Embryonic Stem Cells

Figure 3

Downregulation of TIAR protein levels stimulates the expression of c-myc and TIA-1 in E14 cells. (a) Quantitative RT-PCR analysis of the gene expression of c-myc and TIA-1 in TIAR RNAi E14 cells and pSIREN-RetroQ control E14 cells. E14 cells were used as a control. (b) Western blot analysis of the gene expression of c-myc and TIA-1 in TIAR RNAi E14 cells and pSIREN-RetroQ control E14 cells. (c) Western blot analysis of the gene expression of Oct4, c-myc, and TIAR in undifferentiated TIAR RNAi E14 cells (day 0) and differentiated cell of TIAR RNAi E14 cells (day 4 and day 8). Control: pSIREN-RetroQ control E14 cells; TIAR RNAi: TIAR RNAi E14 cells.

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