Targeted Knockdown of RNA-Binding Protein TIAR for Promoting Self-Renewal and Attenuating Differentiation of Mouse Embryonic Stem Cells

Table 1

Gene expression during EB differentiation in control and TIAR RNAi E14 cells.

	E14 cells	Control	TIAR RNAi
Day 4
Nestin	0.98 ± 0.051	1.0	0.43 ± 0.050
Pax6	1.04 ± 0.070	1.0	0.38 ± 0.065
Flk1	0.95 ± 0.031	1.0	0.42 ± 0.045
Brachyury	0.98 ± 0.066	1.0	0.46 ± 0.035
Sox17	1.06 ± 0.015	1.0	0.44 ± 0.061
Gata6	0.96 ± 0.025	1.0	0.41 ± 0.021
Day 8
Nestin	0.97 ± 0.059	1.0	0.47 ± 0.060
Pax6	1.03 ± 0.045	1.0	0.43 ± 0.067
Flk1	0.99 ± 0.050	1.0	0.46 ± 0.064
Brachyury	0.95 ± 0.061	1.0	0.50 ± 0.035
Sox17	1.05 ± 0.040	1.0	0.37 ± 0.055
Gata6	0.97 ± 0.065	1.0	0.44 ± 0.057
Day 14
Nestin	0.98 ± 0.065	1.0	0.44 ± 0.071
Pax6	1.04 ± 0.076	1.0	0.52 ± 0.030
Flk1	1.04 ± 0.055	1.0	0.46 ± 0.045
Brachyury	0.94 ± 0.045	1.0	0.44 ± 0.059
Sox17	0.96 ± 0.070	1.0	0.48 ± 0.050
Gata6	0.99 ± 0.076	1.0	0.49 ± 0.036

by one-way ANOVA. Control: pSIREN-RetroQ control E14 cells; TIAR RNAi: TIAR RNAi E14 cells.