Transgenic Strategies to Study Podocyte Loss and Regeneration

Figure 3

DTR models and strategies. (a) Schematic representation of a floxed Stop cassette followed by DTR transgene targeted under the Rosa26 promoter. The reporter transgene, activated upon Cre recombinase activity induced by podocin promoter activation, forces podocytes to express the DT receptor. The operator can thus easily induce podocyte cell death in a time- and dose-controlled manner. (b) Quadruple transgenic hNPHS2.rtTa; TetO.Cre; mT/mG; iDTR, in which podocytes can be prompted to turn from red to green while expressing the DTR. Once induced, the authors provoked podocyte death through DT injection [52].

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