Priming Mesenchymal Stem Cells with Endothelial Growth Medium Boosts Stem Cell Therapy for Systemic Arterial Hypertension

Figure 1

Phase-contrast micrographs of MSCs in culture (Panel (a)), showing the typical fibroblastoid morphology. Differentiation of MSCs, which were cultured in osteogenic and adipogenic medium as previously described. Calcium deposited in the extracellular matrix is stained red by Alizarin Red (Panel (b)). Lipid vacuoles are stained orange with Oil Red O (Panel (c)). Magnifications, ×100 (Panels (a)–(c)). (Panel (d)) Immunophenotypic profile of MSCs cultured in conventional medium. Flow cytometry expression of selected molecules (CD11b, CD117(cKit), CD45, CD34, CD31 and CD29) by MSC population.

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