Research Article

Comparisons of Mouse Mesenchymal Stem Cells in Primary Adherent Culture of Compact Bone Fragments and Whole Bone Marrow

Table 1

Cell yields of the first passage from cultured bone marrow and bone fragments.

Cells of the first passage
Whole bone marrowBone fragments

Culture (days/wells)10/310/3
Cell yield (105/well)1.20 ± 0.410.75 ± 0.19
Cell yield (105/mouse)3.50 ± 1.232.25 ± 0.57

Bone marrow and fragments were cultured in 3 wells of 6-well plate from two femurs and two tibiae of a mouse for 10 days. Cells yields of cultured bone marrow were slightly higher than those of cultured fragments ().