Human Bone Marrow-Derived Mesenchymal Stromal Cells Differentially Inhibit Cytokine Production by Peripheral Blood Monocytes Subpopulations and Myeloid Dendritic Cells

Figure 1

Phenotypic characteristics of peripheral blood classical, intermediate, and nonclassical monocytes and mDC. Bivariate dot plot histograms illustrating the phenotypic strategy for the identification of the different monocyte subpopulations and myeloid dendritic cells (mDC) from peripheral blood. Classical monocytes (green events) express CD14 in the absence of CD16, they also show high reactivity for CD45, CD33, IREM-2, and HLA-DR; intermediate monocytes (dark blue events) are characterized as CD14-positive displaying an increasing positivity to CD16, together with a high expression of IREM-2 and positivity for CD45, CD33, and HLA-DR; nonclassical monocytes (light blue events) are CD16-positive with a decreasing expression of CD14, they are highly positive to IREM-2 and CD45, while presenting the lowest CD33 expression among monocytes subpopulations; mDC (pink events) are phenotypically characterized as negative for CD14, CD16, and IREM-2, they present lower expression of CD45 and SSC properties and higher expression of HLA-DR and CD33, compared to monocytes.