Beneficial Effects of Coculturing Synovial Derived Mesenchymal Stem Cells with Meniscus Fibrochondrocytes Are Mediated by Fibroblast Growth Factor 1: Increased Proliferation and Collagen Synthesis

Figure 3

Conditioned medium of SSCs increases proliferation of me-CH through FGF1 signaling pathway. (a) BrdU was stained for proliferating cells at day 3 after forming of aggregates. Pellets of me-CH were cultured in SF medium (serum free medium plus 5 μg/mL normal goat IgG), Con medium (conditioned medium plus 5 μg/mL normal goat IgG), or Con medium + anti-FGF1 (conditioned medium plus 5 μg/mL of goat antibody against FGF1). Positive cells are shown in red, indicated by white arrowheads. Nuclei were counterstained with DAPI (blue). Scale bar = 50 μm. (b) BrdU positive cells were quantified (). Data is shown as mean + standard deviation. Statistical significance was analyzed by one-way ANOVA followed by Dunnett’s test. . . (c) Immunofluorescent staining for FGF1 is performed on SSCs (passage 2). Red fluorescence shows positive staining of FGF1. Nuclei were counterstained with DAPI (blue). Scale bar = 50 μm.

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