Secretome of Olfactory Mucosa Mesenchymal Stem Cell, a Multiple Potential Stem Cell

Figure 4

OM-MSCs differentiate in vitro into osteoblasts, adipocytes, and neurons. Adipogenic, osteogenic, and neuron differentiation was observed and assessed by Oil red O (ORO), alizarin red staining (ARS), or MAP-2 in induction media. (a) Adipocyte cellular morphology in 2 weeks; (b) lipid droplets that showed red color after ORO staining; (c) osteocyte cellular morphology in 3 weeks; (d) ARS that verified the formation of large calcium deposits. (e) Neuron cellular morphology in 12 days; (f) neuron-like cells that can be expressed MAP-2. Nuclei are counterstained with DAPI (blue). Scale bars: (a) and (c) 200 μm; (b) and (d) 100 μm; (e) and (f) 50 μm. DAPI = 4′,6-diamidino-2-phenylindole; MSC = mesenchymal stem cell.

(a)

(b)

(c)

(d)

(e)

(f)