Research Article
Differentiation of Human Adipose Derived Stem Cells into Smooth Muscle Cells Is Modulated by CaMKIIγ
Figure 3
Cell transfection efficiency. CaMKII mRNA (a) and protein (b) expression levels in hASCs transfected with CaMKIIγ and control. (c) qRT-PCR analysis of CaMKIIα, CaMKIIβ, CaMKIIγ, and CaMKIIδ in hASCs transfected with siRNA targeting CaMKIIγ (si-CaMKIIγ) and nontargeting siRNA (si-control) for 48 hours, respectively. (d) Detection of CaMKII isoforms by western blot in hASCs transfected with siRNA targeting CaMKIIγ (si-CaMKIIγ) and nontargeting siRNA (si-control). when (a) versus ad-GFP and (c) si-control.
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