Research Article

Epidermal Cells Expressing Putative Cell Markers in Nonglabrous Skin Existing in Direct Proximity with the Distal End of the Arrector Pili Muscle

Figure 1

α5 integrin and phalloidin staining delineate the distal tips of the arrector pili muscle. Immunofluorescence microscopy showing patterns of phalloidin, expression of α5 integrin, and collagen VII. (a-b) α5 integrin (red) demonstrates a heterogeneous expression in the basal epidermis. (b) Triple immunofluorescence staining for α5 integrin (red), collagen VII (green), and phalloidin (blue) showing marked overlap of α5 integrin and phalloidin in the basal epidermis at the distal tip of the arrector pili muscle. (c) Strong correlation between α5 integrin expressing cells and cells stained with phalloidin. (d) Extensive branching of the APM (phalloidin-stained) as it ascends and attaches to the dermoepidermal junction. Scale bar: 100 μm.
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