Clonal Heterogeneity in the Neuronal and Glial Differentiation of Dental Pulp Stem/Progenitor Cells

Figure 3

Neuronally differentiated high nestin-expressing mDPSCs show immature electrophysiology properties. Current density-voltage relationships of transmembrane K⁺ currents of undifferentiated (a) and neuronally differentiated (b) high nestin-expressing mDPSCs (clone 2) in the absence (○, inlet 1 illustrates exemplar trace of currents,   and  17 cells, resp.) and presence of TEA (1 mM) (■, inlet 2 illustrates exemplar trace of currents, for each differentiation condition). (c) Current density-voltage relationships of transmembrane K⁺ current (○) and Na⁺ currents (▼) of mSTM neurons ( cells). Inlet illustrates exemplar trace of currents. The mean ± SEM current densities at +80 mV of undifferentiated ( pA/pF) and neuronally differentiated mDPSCs ( pA/pF) showed a significant difference in comparison with primary cultured mSTM neurons ( pA/pF): and , respectively. (d) Comparison of capacitances of all three cell types. The mean values ± SEM (□) of undifferentiated mDPSCs () and neuronally differentiated high nestin-expressing mDPSCs (), as well as undifferentiated mDPSCs and P0 mSTM neurons (), were considered as significantly different: and , respectively. ● and ○ are maximal and minimal values, respectively.

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