Dexamethasone Regulates EphA5, a Potential Inhibitory Factor with Osteogenic Capability of Human Bone Marrow Stromal Cells

Figure 3

hBMSCs treated with EphA5-Fc express ALP mRNA at levels similar to those of nontreated hBMSCs. (a) hBMSCs at P5 were left untreated (control) or treated for 7 days with 4 μg/mL Fc or ephrin-Fc. Quantitative analysis of ALP mRNA expression (). The fold change of gene expression was normalized against the expression in cell cultures without Fc treatment. (b) hBMSCs at P1 (left) or P5 (right) were left untreated (control) or treated for 7 days with 0 or 4 μg/mL Fc and EphA5-Fc. Quantitative analysis of ALP mRNA expression (). The fold change of gene expression was normalized against the expression in cell cultures without Fc treatment. Images of wells after ALP staining (upper).

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