Proangiogenic Features of Mesenchymal Stem Cells and Their Therapeutic Applications

Figure 2

Intravital microscope analysis revealed that MSCs behaved like pericytes wrapping around the vessel in a tissue-engineered vascular model. With multiphoton laser scanning microscopy (MPLSM), images were taken at different time points. Lumen formation and blood flow in hMSCs (EGFP+) derived cells were not able to detect (a). On the contrary, implant HUVECs (DsRed+) and hMSCs (EGFP+) in mice, hMSCs (EGFP+) could be found elongate into thin slit structures and coalesced around the HUVEC-derived vessels (b)–(f). Over time, the number of interstitial hMSCs (EGFP+) was decreased, and most of them were associated with blood vessels by day 83 (g). Reprinted with permission from [60].

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