Cytokines TNF-α, IL-6, IL-17F, and IL-4 Differentially Affect Osteogenic Differentiation of Human Adipose Stem Cells

Table 1

Primer sequences for determination of proliferation and osteogenic differentiation of hASCs through PCR. YWHAZ: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta; UBC: ubiquitin C; KI67: proliferation marker; RUNX2: runt-related transcription factor-2; COL1: collagen type 1; Osteocalcin.

Target gene (human)	Oligonucleotide sequences
	Forward	Reverse
YWHAZ	5′ GATGAAGCCATTGCTGAACTTG 3′	5′ CTATTTGTGGGACAGCATGGA 3′
UBC	5′ GCGGTGAACGCCGATGATTAT 3′	5′ TTTGCCTTGACATTCTCGATGG 3′
KI67	5′ CCCTCAGCAAGCCTGAGAA 3′	5′ AGAGGCGTATTAGGAGGCAAG 3′
RUNX2	5′ ATGCTTCATTCGCCTCAC 3′	5′ ACTGCTTGCAGCCTTAAAT 3′
COL1	5′ TCCGGCTCCTGCTCCTCTTA 3′	5′ GGCCAGTGTCTCCCTTG 3′
Osteocalcin	5′AGCCACCGAGACACCATGAGA 3′	5′ CTCCTGAAAGCCGATGTGGTC 3′