Research Article

The Generation of Human Induced Pluripotent Stem Cells from Blood Cells: An Efficient Protocol Using Serial Plating of Reprogrammed Cells by Centrifugation

Figure 4

Generation of iPSCs from CBMCs with the established protocol. (a) Brightfield image of iPSCs generated from CBMCs. Colonies appeared in a similar time frame as PBMC iPSCs. (b) Gene expression of pluripotency markers in N3 from CBMC iPSCs showed similar expression to that of PBMC iPSC N3. (c) Immunocytochemical staining of CBMC iPSC clone N3. Scale bars: 200 μm.
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