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Stem Cells International
Volume 2016 (2016), Article ID 1346521, 7 pages
http://dx.doi.org/10.1155/2016/1346521
Research Article

A Genomic Study of DNA Alteration Events Caused by Ionizing Radiation in Human Embryonic Stem Cells via Next-Generation Sequencing

Nuclear Medicine Division, Department of Radiology and Imaging Sciences, Clinical Center, National Institutes of Health, 9000 Rockville Pike, Bethesda, MD 20892, USA

Received 7 April 2015; Revised 29 May 2015; Accepted 2 June 2015

Academic Editor: Siwanon Jirawatnotai

Copyright © 2016 Van Nguyen et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary Table 1 lists variants that were present in both irradiated and control cells and were either heterozygous (≈50% frequency) or homozygous (≈50% frequency) reflecting the genetic differences (or single nucleotide polymorphism) between the hESC lines tested and reference, but not genomic changes resulted from IR exposure. Identification of all variants was performed using two parallel analysis tools, Ion Torrent Variant Caller plugin version 4.0 (Life Technologies) and NextGENe software version 2.3.4 (SoftGenetics), with appropriate visual inspection of all alignments.

  1. Supplementary Material