Construction of a Dual-Fluorescence Reporter System to Monitor the Dynamic Progression of Pluripotent Cell Differentiation

Figure 1

Alignment of the human, cow, mouse, and pig Oct4 gene and its upstream regulatory sequences (URSs). (a) Pig URS shares relatively low homology with the human (43.11%), cow (38.71%), and mouse (34.66%) gene, respectively. (b) The distribution of CpGs is obviously higher in the porcine Oct4 URS, and three CpG islands appear to exist only in pig but are rarely present in the human, mouse, and cow gene. Red bars at the bottom show the CpG dinucleotide. Light blue shaded regions are the predicted CpG islands. (c) Four highly conserved blocks (CR1, CR2, CR3, and CR4) in pig were found with homologies from 87.19% to 94.88% through pairwise alignments. The right axis indicates the percentage identity within a 100 bp window for each pairwise comparison, ranging from 10% to 100%. Regions sharing greater than 25% identity are shaded and the black horizontal line indicates 50% identity. CR: conserved region. DE: distal enhancer. PE: proximal enhancer. PP: minimal proximal promoter.

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