Construction of a Dual-Fluorescence Reporter System to Monitor the Dynamic Progression of Pluripotent Cell Differentiation

Figure 4

F9 cells and P19 cells exhibit completely different fluorescence signals. (a) The transgenic ESC-like F9 cells show a higher red fluorescence, while green fluorescence was primarily detected in EpiSC-like P19 cells. The negative control cell line, MEFs, exhibited neither green nor red fluorescence expression. (b) Quantitative analysis of the fluorescence intensity in a same cell clone. The fluorescence intensity of the pictures was converted to corrected total cell fluorescence (CTCF) by ImageJ 2x as previously described [35]. CTCF = integrated density  −  (area of selected cell × mean fluorescence of background readings). Data are represented as the mean ± SEM. indicates value < 0.05 versus normal. indicates value < 0.05 versus normal. Similar results were observed in more than two independent experiments. Scale bars indicate 20 µm.

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