Construction of a Dual-Fluorescence Reporter System to Monitor the Dynamic Progression of Pluripotent Cell Differentiation

Figure 5

Classifying mESCs from a heterogenous population by dual-fluorescence signals. (a) When cultured in vitro, most ESC colonies exhibit a three-dimensional dome-like morphology (1), but a fraction of ESCs may spontaneously differentiate into flattened (2, 3) or rosette-like structures (4). (b) Four cell types were identified in transgenic mESCs. A relatively higher level of red fluorescence was detected in Type I cells. A similar level of green and red fluorescence was detected in Type II cells. A relatively higher level of green fluorescence was detected in Type III cells. Neither green nor red fluorescence is detected in Type IV cells. CTCF: corrected total cell fluorescence. (c) Quantitative analysis of the fluorescence intensity in the same cell clone. The fluorescence signal was scanned by ImageJ and converted into numeric data for comparison of cell types. Scale bars indicate 50 µm. Data are represented as the mean ± SEM. indicates value < 0.05 versus normal. indicates value < 0.05 versus normal. Similar results were observed in more than two independent experiments.