Research Article
Disrupted Endothelial Cell Layer and Exposed Extracellular Matrix Proteins Promote Capture of Late Outgrowth Endothelial Progenitor Cells
Figure 1
Representative phase-contrast live cell images of interaction of LO-EPC with 3 × 104 subconfluent (a) and 6 × 104 confluent (b) HAEC monolayers under shear stress 0.7 dyn/cm2. 4 × 105 LO-EPC were perfused for 4 min. Arrows indicated adherent LO-EPC which only adhered paracellularly at junctional regions of discontinuity between two cells in subconfluent HAEC monolayer. Scale bar 30 μm. After adhesion LO-EPC spread and establish cell-cell interaction (c). LO-EPC were labelled with DiI-Ac-LDL shown red. Scale bar 60 μm. VE-Cadherin staining revealed the formation of lateral junction between LO-EPC and HAEC (d). LO-EPC were labelled with DiI-Ac-LDL shown red and VE-Cadherin expression shown green.
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