Disrupted Endothelial Cell Layer and Exposed Extracellular Matrix Proteins Promote Capture of Late Outgrowth Endothelial Progenitor Cells

Figure 2

Interaction of LO-EPC with activated HAEC. Surface expression of adhesion molecules in HAEC after stimulation with 0.05 ng/mL TNFα (a). Surface expressions of E-selectin, ICAM-1, and VCAM-1 were quantified using flow cytometry. The graph shows relative Mean Fluorescence Intensity (MFI) normalised to untreated cells and represents the mean ± SE of three experiments. . Activation of HAEC did not increase the interaction with LO-EPC (b). 4 × 10⁵ LO-EPC were perfused to 3 × 10⁴ HAEC for 4 min and the data represented as mean ± SE of three experiments.

(a)

(b)