Disrupted Endothelial Cell Layer and Exposed Extracellular Matrix Proteins Promote Capture of Late Outgrowth Endothelial Progenitor Cells

Figure 6

LO-EPC (4 × 10⁵) were perfused over fibronectin-coated Ibidi slides and time lapse imaging was used to visualise and record LO-EPC adhesion. Representative still images show three adherent cells after initial capture (a, b) after 4 min of perfusion (0.7 dyn/cm²). Scale bar represents 30 μm. Adherent LO-EPC form lateral adhesion junction (c). LO-EPC labelled with DiI-Ac-LDL shown red and VE-Cadherin expression shown green. Scale bar 20 μm. 24 hours after adhesion to fibronectin, LO-EPC spread and proliferate (d). Scale bar represents 60 μm. The adherent LO-EPC effectively cover a fibronectin-coated surface (e). Scale bar represents 110 μm.