Research Article
GDNF Enhances Therapeutic Efficiency of Neural Stem Cells-Based Therapy in Chronic Experimental Allergic Encephalomyelitis in Rat
Figure 1
The generation and characterization of the neurosphere in vitro. (a) Cultured neurospheres derived from cerebral tissue of newborn rats at culture of 7 d. (b) Immunocytochemical staining of nestin in neurospheres. (c) Neurospheres infected by GDNF recombinant adenovirus for 2 days showed green fluorescence under fluorescence microscope. (d) RT-PCR showed higher level of GDNF mRNA in the GDNF/NSCs than NSCs. (e) Quantitative analysis of the percentages of NSCs and GDNF/NSCs differentiated into each type of neural cells (mean ± SD, ). with NSCs group, . (f–k) Immunostaining showed, respectively, MAP2, GalC, and GFAP-positive cells from neurospheres. (f) MAP2+ cells from NSCs; (g) MAP2+ cells from GDNF/NSCs; (h) GalC+ cells from NSCs; (i) GalC+ cells from GDNF/NSCs; (j) GFAP+ cells from NSCs; (k) GFAP+ cells from GDNF/NSCs; (a–c) bar = 50 μm; (f–k) bar = 75 μm.
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