GDNF Enhances Therapeutic Efficiency of Neural Stem Cells-Based Therapy in Chronic Experimental Allergic Encephalomyelitis in Rat

Figure 3

Attenuated inflammation and more myelin sheath in GDNF/NSCs compared to NSCs in EAE rats. (a–c) HE staining showed inflammatory infiltrates of the cerebral parenchyma in control, NSCs, and GDNF/NSCs groups, respectively. The control group showed much more infiltration with inflammatory cells. The transplanted groups displayed a reduction in the amount and size of the inflammatory infiltration, especially for the GDNF/NSC transplanted group. (d) Quantitative analysis of the amount of infiltration per mm² (mean ± SD, ). with control group, . (e) Quantitative analysis of the number of cells per infiltration (mean ± SD, ). with control group, . ^#Compared with NSCs group, . (f–h) LFB staining showed myelin sheath of the corpus callosum in control, NSCs, and GDNF/NSCs groups, respectively. Control group showed marked sparser and thinner myelin sheath compared with transplanted groups. In particular, GDNF/NSCs groups displayed more myelinated fibers than NSCs group. (i) Quantitative analysis of LFB density (mean ± SD, ). with control group, ; ^#compared with NSCs group, . Bar = 100 μm.

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