Delta-Like-1 Changes the Immunomodulatory Property of OP9 Cells

Figure 5

OP9 and OP9DL1 inhibit proliferation of mature T cells in vitro. CD3⁺ T cells were isolated from murine spleens with CD3ε MicroBead Kits and labeled with CFSE. T cells were stimulated with PMA (50 ng/mL) plus ionomycin (1 μg/mL) for 24 h and then cultured alone or with OP9 or OP9DL1 at different ratios (OP9 or OP9DL1 cells to T cells). After 36 h, all of the cells were analyzed via microscope (a) and flow cytometry (b) for T cell proliferation as indicated by the reduction in CFSE intensity. Data are representative of three independent experiments.

(a)

(b)