Optimized Stem Cell Detection Using the DyeCycle-Triggered Side Population Phenotype

Figure 5

Evaluation of cell death induction by pharmacological inhibitors of drug transporter activity. A2780V cells (10⁶ cells/mL) were stained with 10 μM DCV. Control aliquots containing different concentrations of inhibitor (either verapamil or fumitremorgin C) were prepared thereof and analysed for cell viability using 7-AAD staining. Verapamil induces significant cell death already at the commonly used dose of 50 μM, whereas the ABCG2-specific inhibitor fumitremorgin C leaves the cells virtually unaffected.