Optimized Stem Cell Detection Using the DyeCycle-Triggered Side Population Phenotype

Figure 7

Detection of DCV-SP cells using various flow cytometric instruments. A2780V cells were stained with 10 μM DCV (10⁶ cells/mL) and analysed on the indicated flow cytometers within one hour. Note that all of the investigated instruments facilitate reasonable discrimination of SP cells, even though certain instruments separate particularly well, for instance, LSRFortessa.