In Vitro Osteogenic Potential of Green Fluorescent Protein Labelled Human Embryonic Stem Cell-Derived Osteoprogenitors

Figure 4

Characterization of osteogenic differentiation of hESC-OPs (GFP+) by real-time RT-PCR. The transcript levels were normalized to respective β-Actin levels and to hESC (GFP+) cells. Values represent the means ± SD of three experiments ( versus respective controls). hESC-OPs (GFP+) were differentiated for 4 weeks in the presence of osteogenic factors (OD). Day 0 hESC-OPs (GFP+) and cells differentiated for 4 weeks without osteogenic factors were used as controls.