Mutation-Specific Phenotypes in hiPSC-Derived Cardiomyocytes Carrying Either Myosin-Binding Protein C Or α-Tropomyosin Mutation for Hypertrophic Cardiomyopathy

Figure 1

Characterization of UTA.13602.HCMT cell line. (a) The hiPSCs formed colonies expressing Nanog, OCT4, SOX2, TRA-1-60, and TRA-1-81. Scale bars: 200 μm. (b) The virally transferred Sendai exogenes, exo-OCT4 (483 bp), exo-KLF4 (410 bp), exo-SOX2 (451 bp), and exo-c-MYC (532 bp), were silenced in the hiPSCs. + indicates positive controls, for which RNA was extracted from cells 1 week after transduction. hiPSCs expressed endogenous Nanog (287 bp), OCT4 (144 bp), REX1 (306 bp), SOX2 (151 bp), and c-MYC (328 bp). GAPDH (302 bp) was used as a housekeeping control. (c) The hiPSC line was karyotypically normal, 46 XX. (d) The pluripotency of hiPSCs was confirmed by in vivo teratoma assay, in which hiPSCs formed all three germ layers (mesoderm, endoderm, and ectoderm).

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