Wnt5a Increases Properties of Lung Cancer Stem Cells and Resistance to Cisplatin through Activation of Wnt5a/PKC Signaling Pathway

Figure 5

An inhibition of PKC signaling reduces the fraction of ALDH-positive in cisplatin-resistant lung cancer cells. A549/DDP cells were cultured in medium containing Wnt5a, PKC inhibitor GF109203X, and/or cisplatin for 24 hours; the cells were then harvested for ALDH staining and flow cytometric analysis. (a) Representative cytometric plots showed the positive fractions of ALDH-positive cells treated with indicated conditions. (b) A quantitative analysis of flow cytometry data showed Wnt5a slightly increased ALDH-positive cell fraction in A549/DDP cells in the absence of cisplatin, and an inhibition of PKC signaling by GF109203 significantly reduced the frequency of ALDH-cells in the presence of cisplatin. Compared to the corresponding non-GF109203X-treated group and other groups, represents . All data are represented as the mean ± SD of three independent triplicated experiments (). GF: GF109203X.

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