Wnt5a Increases Properties of Lung Cancer Stem Cells and Resistance to Cisplatin through Activation of Wnt5a/PKC Signaling Pathway

Figure 6

Wnt5a activates Wnt/calcium/PKC signaling in A549 and A549/DDP cells. A549 and A549/DDP were exposed to Wnt5a, PKC inhibitor GF109203X, and/or cisplatin during the culturing. The cells were then harvested for analyzing the expression of key components of Wnt/calcium/PKC signaling cascade. (a) Representative images of immunoblotting analysis in A549 cells. (b) The relative levels of proteins detected as evaluated by a densitometric analysis for proteins of interest of A549 cells in (a). (c) Representative images of immunoblotting analysis in A549/DDP cells. (d) The relative levels of proteins detected as evaluated by a densitometric analysis for proteins of interest of A549/DDP cells in (c). p-PKC: phosphorylated PKC.