Wnt5a Increases Properties of Lung Cancer Stem Cells and Resistance to Cisplatin through Activation of Wnt5a/PKC Signaling Pathway

Figure 7

An inhibition of Wnt/calcium/PKC signaling induced apoptosis in lung cancer cells. A549 and A549/DDP cells were cultured in medium containing Wnt5a, PKC inhibitor GF109203X, and/or cisplatin. Cell apoptosis analyzed by a cytometric assay using an Annexin V-FITC/propidium iodide (PI) apoptosis detection kit. (a) Fractions of apoptotic cells in A549 cells treated with indicated conditions. (b) Fractions of apoptotic cells in A549/DDP cells treated with indicated conditions. An exposure of A549/DDP cells to Wnt5a showed a decreased cell apoptosis, but an inhibition of Wnt/calcium/PKC signaling by GF109203 increased the apoptosis in both A549 and A549/DDP cells, regardless of the presence of Wnt5a or cisplatin. Compared to the Control-CM group, represents . Compared to the corresponding non-GF109203X-treated group, represents . All data are represented as the mean ± SD of three independent triplicated experiments (). GF: GF109203X.

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