Wnt5a Increases Properties of Lung Cancer Stem Cells and Resistance to Cisplatin through Activation of Wnt5a/PKC Signaling Pathway

Figure 8

Apoptosis related proteins determined by an immunoblotting analysis. A549 and A549/DDP cells were cultured in medium containing Wnt5a, PKC inhibitor GF109203X, and/or cisplatin for 24 hours; the cells were then harvested for immunoblotting analysis of indicated proteins. (a) Representative images of immunoblotting analysis in A549 cells. (b) The relative levels of proteins detected as evaluated by a densitometric analysis for proteins of interest of A549 cells in (a). (c) Representative images of immunoblotting analysis in A549/DDP cells. (d) The relative levels of proteins detected as evaluated by a densitometric analysis for proteins of interest of A549/DDP cells in (c). An exposure of A549/DDP cells to Wnt5a reduced the expression of proapoptotic proteins (caspase 3, Bax, and AIF) but increased the expression of antiapoptotic protein Bcl-2 in lung cancer cells. Cas3: caspase 3.