Electrical Stimulation Promotes Cardiac Differentiation of Human Induced Pluripotent Stem Cells

Figure 6

Electrophysiological properties of cardiomyocytes derived from iPS(Foreskin)-2 cell line. (a) Beating EBs at day 14 after plating were cycling calcium. Intensity of fluorescence has been normalized with background. (b) A representative image of a microelectrode array with beating pieces and the extracellular field potential recorded. (c) The percentage change in beating rate and (d) the cFPD of cardiomyocytes from control and electrically stimulated (ES, 200 mV/mm, 5 min) groups treated with isoproterenol hydrochloride (isoprenaline, 1–1000 nM) or carbamylcholine (carbachol, 1–1000 nM) ( independent experiments). (e) Quantitative RT-PCR analysis for the expression of muscarinic receptor 2 (CHM2), β-adrenergic receptor-1 (ADRB1), and β-adrenergic receptor-2 (ADRB2) in control and electrically stimulated beating EBs (–7 independent experiments). ES: electrically stimulated and undiff: undifferentiated iPSCs. Data are expressed as mean ± SEM. , , and by one-way ANOVA with the Bonferroni post hoc test.

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