The Controversy, Challenges, and Potential Benefits of Putative Female Germline Stem Cells Research in Mammals

Figure 1

The isolation and identification of putative FGSCs. (a) H&E stained from histological ovaries sections: putative FGSCs have been considered to be mainly localized in ovarian cortex. (b) IHC results: putative FGSCs were detected by stem cell marker Oct-4 antibody. (c) Putative FGSCs were isolated by primary culture method. Arrows: the freshly isolated putative FGSCs. (d) Arrows: the isolated MVH⁺ putative FGSCs. (e) Green: the isolated BrdU⁺ cells. (f) The merger of (d) and (e) by dual immunofluorescence.

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