PAHs Target Hematopoietic Linages in Bone Marrow through Cyp1b1 Primarily in Mesenchymal Stromal Cells but Not AhR: A Reconstituted In Vitro Model

Figure 1

Comparison of progenitor activity after in vivo PAH treatment in mice with normal (WT) and diminished () AhR activity. (a) Diagram of in vivo treatments and isolation points for colony forming unit (CFU) analyses. Mice were treated with DMBA or BP (50 mg/kg) or an equivalent volume of solvent control (DMSO) via a single IP injection. Bone marrow cells (BMC) were isolated 6 and 48 hours after treatment and analyzed for PreB CFU activity. (b) Comparison of PreB CFU activity in WT and BMC 6 and 48 hours after PAH administration (50 mg/kg). Significance () indicates PAH suppression from untreated control or between PAH treatments at the same time point within a genotype, as indicated.

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